University of Eldoret, Kenya
Title: Intrinsic antibiotic resistance of salt and draught tolerant rhizobia bacteria
Jared 24yeras old from Kenya graduated from university of Eldoret in 2017 where he did a Bachelor of Science in microbiology. Before graduating He did a research project on the intrinsic antibiotic resistance of salt and drought tolerant rhizobia bacteria. He is particularly passionate about microbiology research innovation and access to most research activities. Jared was attached at Kenya bureau of standards where he acquired a lot of microbiology skills.
Salt, drought and antibiotics secreted by rhizosphere microorganisms in the soil affects the ability of rhizobia to effectively modulate groundnut [legumes]. The objectives of this study were to (1) screen groundnut rhizobia for salt tolerance; (2) determine the performance of those salt tolerant rhizobia under drought stress and (3) evaluate the response of those salt and drought tolerant rhizobia to antibiotics. Seventeen groundnut rhizobia isolates were screened for salt and drought tolerance using sodium chloride salt at 0%,1%,3%,4% and 5% and polyethylene glycon[PEG] at 10%,20% and 30% respectively. Antibiotic resistance of those salt and drought tolerant rhizobia was screened using eight different types of antibiotics impregnated on the antibiotic disc. Groundnut rhizobia varied in response to salt stress. Isolates A01, A05, BIOFIX, D01, K01, F05 and G06 grew at 5% of Sodium chloride. , a total of seven isolates grew at 5%, Five of the salt tolerant rhizobia isolates A01, A05, F04, F05 and BIOFIX grew on 30% concentration of PEG. Salt and drought tolerant rhizobia varied significantly in response to antibiotics. Five isolates A01, A05, G06, K01 and NO1 were resistant to Nalidixic acid, Gentamycin, Norfloxacin, Ofloxacin and Ceftriaxone. Phosphate solubilizing groundnut rhizobia showed variation in response to salt, drought and antibiotic stresses. The promising isolates that tolerated wide environmental stresses should be tested under greenhouse conditions to improve groundnut and other legumes production. Upon validation in the greenhouse they should be distributed to farmers as bio fertilizers.